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Analysing gene expression [[electronic resource] ] : a handbook of methods: possibilities and pitfalls / / edited by Stefan Lorkowski and Paul Cullen
| Analysing gene expression [[electronic resource] ] : a handbook of methods: possibilities and pitfalls / / edited by Stefan Lorkowski and Paul Cullen |
| Pubbl/distr/stampa | Weinheim ; ; New York, : Wiley-VCH, c2003 |
| Descrizione fisica | 1 online resource (984 p.) |
| Disciplina |
572.8/65
572.865 |
| Altri autori (Persone) |
LorkowskiStefan
CullenPaul, Dr. |
| Soggetto topico | Gene expression |
| Soggetto genere / forma | Electronic books. |
| ISBN |
1-280-52042-6
9786610520428 3-527-60533-9 3-527-60149-X |
| Formato | Materiale a stampa  |
| Livello bibliografico | Monografia |
| Lingua di pubblicazione | eng |
| Nota di contenuto |
Analysing Gene Expression A Handbook of Methods Possibilities and Pitfalls; Foreword; Preface; Contents; List of Contributors; Volume 1; 1 Basic concepts of gene expression; 1.1 Introduction; 1.2 Basics of transcription and translation in the cell; 1.2.1 Introduction; 1.2.2 Transcription; 1.2.3 Translation; 1.2.4 Summary; 1.3 Regulation of transcription; 1.3.1 Introduction; 1.3.2 mRNA expression profiles - the transcriptome; 1.3.3 Protein expression profiles - the proteome; 1.3.4 Interaction between genes and proteins - the interactome; 1.3.5 The transcription machinery and core promoters
1.3.6 Regulatory promoters1.3.7 Enhancers; 1.3.8 Locus control regions; 1.3.9 Matrix attachment regions; 1.3.10 Insulators; 1.3.11 RIDGEs - Regions of increased gene expression; 1.3.12 Enhanceosomes; 1.3.13 Chromatin; 1.3.14 Silencer elements; 1.3.15 Transcription factors, repressors and co-repressors; 1.3.16 Epigenetics; 1.3.17 Summary and conclusions; 1.4 Post-transcriptional regulation; 1.4.1 Introduction; 1.4.2 Regulation of RNA stability and degradation; 1.4.3 Regulation of transcription elongation; 1.4.3.1 Introduction; 1.4.3.2 Regulation of transcription elongation in prokaryotes 1.4.3.3 Regulation of transcription elongation in eukaryotes1.4.3.4 Conclusions; 1.4.4 Differential/alternative pre-mRNA splicing; 1.4.5 Trans-RNA splicing; 1.4.6 Regulation of mRNA transport; 1.4.7 Directed intracellular mRNA localisation; 1.4.8 Regulation of mRNA polyadenylation; 1.4.9 Antisense RNA; 1.4.10 RNA editing; 1.4.11 Summary and conclusions; 1.5 Post-translational modification of proteins; 1.5.1 Introduction; 1.5.2 Proteolytic cleavage of proteins; 1.5.3 Acylation; 1.5.4 Prenylation; 1.5.5 Methylation; 1.5.6 Sulphation; 1.5.7 Phosphorylation; 1.5.8 Ubiquitination 1.5.9 Glycosylation1.5.10 Conclusions; 1.6 Correlation of mRNA and protein expression; 1.6.1 Introduction; 1.6.2 Levels of mRNA and protein expression: correlations and discrepancies; 1.6.3 Conclusions; 1.7 Housekeeping genes, internal and external standards; 1.7.1 What are housekeeping genes?; 1.7.2 Survey of the most important housekeeping genes; 1.7.2.1 Glyceraldehyde-3-phosphate dehydrogenase; 1.7.2.2 β-Actin; 1.7.3 Other commonly used housekeeping genes; 1.7.3.1 Ribosomal RNA (rRNA); 1.7.4 New identified 'maintenance genes'; 1.7.5 Methods of quantification 1.7.5.1 Normalisation (via internal standards)1.7.5.2 Standard curves (via external standards); 1.7.5.3 Alternative methods (via internal standards); 1.7.6 Summary; 1.8 Classification of differential gene expression technologies; 1.8.1 Introduction; 1.8.2 From single genes to transcriptomes; 1.8.3 Methods of classification; 1.8.4 Conclusions; 1.9 Summary; 1.10 References; 2 Sample preparation and supplementary tools; 2.1 Introduction; 2.2 Preparation of cells and tissues; 2.2.1 Immunopurification of cells; 2.2.2 Differential-velocity centrifugation/countercurrent elutriation 2.2.3 Surface affinity chromatography |
| Record Nr. | UNINA-9910146242103321 |
| Weinheim ; ; New York, : Wiley-VCH, c2003 | ||
| Materiale a stampa | ||
| Lo trovi qui: Univ. Federico II | ||
| ||
Analysing gene expression [[electronic resource] ] : a handbook of methods: possibilities and pitfalls / / edited by Stefan Lorkowski and Paul Cullen
| Analysing gene expression [[electronic resource] ] : a handbook of methods: possibilities and pitfalls / / edited by Stefan Lorkowski and Paul Cullen |
| Pubbl/distr/stampa | Weinheim ; ; New York, : Wiley-VCH, c2003 |
| Descrizione fisica | 1 online resource (984 p.) |
| Disciplina |
572.8/65
572.865 |
| Altri autori (Persone) |
LorkowskiStefan
CullenPaul, Dr. |
| Soggetto topico | Gene expression |
| ISBN |
1-280-52042-6
9786610520428 3-527-60533-9 3-527-60149-X |
| Formato | Materiale a stampa |
| Livello bibliografico | Monografia |
| Lingua di pubblicazione | eng |
| Nota di contenuto |
Analysing Gene Expression A Handbook of Methods Possibilities and Pitfalls; Foreword; Preface; Contents; List of Contributors; Volume 1; 1 Basic concepts of gene expression; 1.1 Introduction; 1.2 Basics of transcription and translation in the cell; 1.2.1 Introduction; 1.2.2 Transcription; 1.2.3 Translation; 1.2.4 Summary; 1.3 Regulation of transcription; 1.3.1 Introduction; 1.3.2 mRNA expression profiles - the transcriptome; 1.3.3 Protein expression profiles - the proteome; 1.3.4 Interaction between genes and proteins - the interactome; 1.3.5 The transcription machinery and core promoters
1.3.6 Regulatory promoters1.3.7 Enhancers; 1.3.8 Locus control regions; 1.3.9 Matrix attachment regions; 1.3.10 Insulators; 1.3.11 RIDGEs - Regions of increased gene expression; 1.3.12 Enhanceosomes; 1.3.13 Chromatin; 1.3.14 Silencer elements; 1.3.15 Transcription factors, repressors and co-repressors; 1.3.16 Epigenetics; 1.3.17 Summary and conclusions; 1.4 Post-transcriptional regulation; 1.4.1 Introduction; 1.4.2 Regulation of RNA stability and degradation; 1.4.3 Regulation of transcription elongation; 1.4.3.1 Introduction; 1.4.3.2 Regulation of transcription elongation in prokaryotes 1.4.3.3 Regulation of transcription elongation in eukaryotes1.4.3.4 Conclusions; 1.4.4 Differential/alternative pre-mRNA splicing; 1.4.5 Trans-RNA splicing; 1.4.6 Regulation of mRNA transport; 1.4.7 Directed intracellular mRNA localisation; 1.4.8 Regulation of mRNA polyadenylation; 1.4.9 Antisense RNA; 1.4.10 RNA editing; 1.4.11 Summary and conclusions; 1.5 Post-translational modification of proteins; 1.5.1 Introduction; 1.5.2 Proteolytic cleavage of proteins; 1.5.3 Acylation; 1.5.4 Prenylation; 1.5.5 Methylation; 1.5.6 Sulphation; 1.5.7 Phosphorylation; 1.5.8 Ubiquitination 1.5.9 Glycosylation1.5.10 Conclusions; 1.6 Correlation of mRNA and protein expression; 1.6.1 Introduction; 1.6.2 Levels of mRNA and protein expression: correlations and discrepancies; 1.6.3 Conclusions; 1.7 Housekeeping genes, internal and external standards; 1.7.1 What are housekeeping genes?; 1.7.2 Survey of the most important housekeeping genes; 1.7.2.1 Glyceraldehyde-3-phosphate dehydrogenase; 1.7.2.2 β-Actin; 1.7.3 Other commonly used housekeeping genes; 1.7.3.1 Ribosomal RNA (rRNA); 1.7.4 New identified 'maintenance genes'; 1.7.5 Methods of quantification 1.7.5.1 Normalisation (via internal standards)1.7.5.2 Standard curves (via external standards); 1.7.5.3 Alternative methods (via internal standards); 1.7.6 Summary; 1.8 Classification of differential gene expression technologies; 1.8.1 Introduction; 1.8.2 From single genes to transcriptomes; 1.8.3 Methods of classification; 1.8.4 Conclusions; 1.9 Summary; 1.10 References; 2 Sample preparation and supplementary tools; 2.1 Introduction; 2.2 Preparation of cells and tissues; 2.2.1 Immunopurification of cells; 2.2.2 Differential-velocity centrifugation/countercurrent elutriation 2.2.3 Surface affinity chromatography |
| Record Nr. | UNINA-9910830158703321 |
| Weinheim ; ; New York, : Wiley-VCH, c2003 | ||
| Materiale a stampa | ||
| Lo trovi qui: Univ. Federico II | ||
| ||
Analysing gene expression [[electronic resource] ] : a handbook of methods: possibilities and pitfalls / / edited by Stefan Lorkowski and Paul Cullen
| Analysing gene expression [[electronic resource] ] : a handbook of methods: possibilities and pitfalls / / edited by Stefan Lorkowski and Paul Cullen |
| Pubbl/distr/stampa | Weinheim ; ; New York, : Wiley-VCH, c2003 |
| Descrizione fisica | 1 online resource (984 p.) |
| Disciplina |
572.8/65
572.865 |
| Altri autori (Persone) |
LorkowskiStefan
CullenPaul, Dr. |
| Soggetto topico | Gene expression |
| ISBN |
1-280-52042-6
9786610520428 3-527-60533-9 3-527-60149-X |
| Formato | Materiale a stampa |
| Livello bibliografico | Monografia |
| Lingua di pubblicazione | eng |
| Nota di contenuto |
Analysing Gene Expression A Handbook of Methods Possibilities and Pitfalls; Foreword; Preface; Contents; List of Contributors; Volume 1; 1 Basic concepts of gene expression; 1.1 Introduction; 1.2 Basics of transcription and translation in the cell; 1.2.1 Introduction; 1.2.2 Transcription; 1.2.3 Translation; 1.2.4 Summary; 1.3 Regulation of transcription; 1.3.1 Introduction; 1.3.2 mRNA expression profiles - the transcriptome; 1.3.3 Protein expression profiles - the proteome; 1.3.4 Interaction between genes and proteins - the interactome; 1.3.5 The transcription machinery and core promoters
1.3.6 Regulatory promoters1.3.7 Enhancers; 1.3.8 Locus control regions; 1.3.9 Matrix attachment regions; 1.3.10 Insulators; 1.3.11 RIDGEs - Regions of increased gene expression; 1.3.12 Enhanceosomes; 1.3.13 Chromatin; 1.3.14 Silencer elements; 1.3.15 Transcription factors, repressors and co-repressors; 1.3.16 Epigenetics; 1.3.17 Summary and conclusions; 1.4 Post-transcriptional regulation; 1.4.1 Introduction; 1.4.2 Regulation of RNA stability and degradation; 1.4.3 Regulation of transcription elongation; 1.4.3.1 Introduction; 1.4.3.2 Regulation of transcription elongation in prokaryotes 1.4.3.3 Regulation of transcription elongation in eukaryotes1.4.3.4 Conclusions; 1.4.4 Differential/alternative pre-mRNA splicing; 1.4.5 Trans-RNA splicing; 1.4.6 Regulation of mRNA transport; 1.4.7 Directed intracellular mRNA localisation; 1.4.8 Regulation of mRNA polyadenylation; 1.4.9 Antisense RNA; 1.4.10 RNA editing; 1.4.11 Summary and conclusions; 1.5 Post-translational modification of proteins; 1.5.1 Introduction; 1.5.2 Proteolytic cleavage of proteins; 1.5.3 Acylation; 1.5.4 Prenylation; 1.5.5 Methylation; 1.5.6 Sulphation; 1.5.7 Phosphorylation; 1.5.8 Ubiquitination 1.5.9 Glycosylation1.5.10 Conclusions; 1.6 Correlation of mRNA and protein expression; 1.6.1 Introduction; 1.6.2 Levels of mRNA and protein expression: correlations and discrepancies; 1.6.3 Conclusions; 1.7 Housekeeping genes, internal and external standards; 1.7.1 What are housekeeping genes?; 1.7.2 Survey of the most important housekeeping genes; 1.7.2.1 Glyceraldehyde-3-phosphate dehydrogenase; 1.7.2.2 β-Actin; 1.7.3 Other commonly used housekeeping genes; 1.7.3.1 Ribosomal RNA (rRNA); 1.7.4 New identified 'maintenance genes'; 1.7.5 Methods of quantification 1.7.5.1 Normalisation (via internal standards)1.7.5.2 Standard curves (via external standards); 1.7.5.3 Alternative methods (via internal standards); 1.7.6 Summary; 1.8 Classification of differential gene expression technologies; 1.8.1 Introduction; 1.8.2 From single genes to transcriptomes; 1.8.3 Methods of classification; 1.8.4 Conclusions; 1.9 Summary; 1.10 References; 2 Sample preparation and supplementary tools; 2.1 Introduction; 2.2 Preparation of cells and tissues; 2.2.1 Immunopurification of cells; 2.2.2 Differential-velocity centrifugation/countercurrent elutriation 2.2.3 Surface affinity chromatography |
| Record Nr. | UNINA-9910841895203321 |
| Weinheim ; ; New York, : Wiley-VCH, c2003 | ||
| Materiale a stampa | ||
| Lo trovi qui: Univ. Federico II | ||
| ||
Thorps in a changing landscape [[electronic resource] /] / by Paul Cullen, Richard Jones and David N. Parsons
| Thorps in a changing landscape [[electronic resource] /] / by Paul Cullen, Richard Jones and David N. Parsons |
| Autore | Cullen Paul |
| Pubbl/distr/stampa | Hatfield, : Hertfordshire University Press, c2011 |
| Descrizione fisica | 1 online resource (243 p.) |
| Altri autori (Persone) |
JonesRichard
ParsonsDavid N |
| Collana | Explorations in local and regional history |
| Soggetto topico |
Names, Geographical - England
Villages - Great Britain |
| Soggetto genere / forma | Electronic books. |
| ISBN | 1-907396-15-2 |
| Formato | Materiale a stampa |
| Livello bibliografico | Monografia |
| Lingua di pubblicazione | eng |
| Record Nr. | UNINA-9910453389103321 |
Cullen Paul
|
||
| Hatfield, : Hertfordshire University Press, c2011 | ||
| Materiale a stampa | ||
| Lo trovi qui: Univ. Federico II | ||
| ||
Thorps in a changing landscape [[electronic resource] /] / by Paul Cullen, Richard Jones and David N. Parsons
| Thorps in a changing landscape [[electronic resource] /] / by Paul Cullen, Richard Jones and David N. Parsons |
| Autore | Cullen Paul |
| Pubbl/distr/stampa | Hatfield, : Hertfordshire University Press, c2011 |
| Descrizione fisica | 1 online resource (243 p.) |
| Altri autori (Persone) |
JonesRichard
ParsonsDavid N |
| Collana | Explorations in local and regional history |
| Soggetto topico |
Names, Geographical - England
Villages - Great Britain |
| ISBN | 1-907396-15-2 |
| Formato | Materiale a stampa |
| Livello bibliografico | Monografia |
| Lingua di pubblicazione | eng |
| Record Nr. | UNINA-9910790783403321 |
|
Cullen Paul
|
||
| Hatfield, : Hertfordshire University Press, c2011 | ||
| Materiale a stampa | ||
| Lo trovi qui: Univ. Federico II | ||
| ||
Thorps in a changing landscape [[electronic resource] /] / by Paul Cullen, Richard Jones and David N. Parsons
| Thorps in a changing landscape [[electronic resource] /] / by Paul Cullen, Richard Jones and David N. Parsons |
| Autore | Cullen Paul |
| Pubbl/distr/stampa | Hatfield, : Hertfordshire University Press, c2011 |
| Descrizione fisica | 1 online resource (243 p.) |
| Altri autori (Persone) |
JonesRichard
ParsonsDavid N |
| Collana | Explorations in local and regional history |
| Soggetto topico |
Names, Geographical - England
Villages - Great Britain |
| ISBN | 1-907396-15-2 |
| Formato | Materiale a stampa |
| Livello bibliografico | Monografia |
| Lingua di pubblicazione | eng |
| Record Nr. | UNINA-9910807241703321 |
|
Cullen Paul
|
||
| Hatfield, : Hertfordshire University Press, c2011 | ||
| Materiale a stampa | ||
| Lo trovi qui: Univ. Federico II | ||
| ||